He is most widely known for his efforts in creating the plant tissue culture medium known as Murashige and Skoog medium.

Murashige and Skoog medium (Plant cell culture medium)

Ethylenediamine (Edamine) is a common organic additive to the plant in vitro culture Murashige and Skoog medium.

There are several types of basal salt mixtures used in plant tissue culture, but most notably modified Murashige and Skoog medium, White's medium, and woody plant medium.

Murashige and Skoog medium or (MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture.

Now referred to as Murashige and Skoog medium, the final paper (Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures.

While in general, two main types of basal media are the most commonly used for embryo rescue studies, i.e. Murashige and Skoog medium (MS) and Gamborg's B-5 media (Bridgen, 1994), the composition of the media will vary in terms of the concentrations of media supplements required.