amplicon

Multiple runs of a single amplicon were found to be highly reproducible.

This region that is amplified is known as the amplicon.

Optimal Tc must be measured and determined for each amplicon.

Both of these parameters are a function of the amplicon sequence.

Various detection and capture steps are involved in each approach to assess the amplification product, or amplicon.

Then each amplicon is sequenced with the pyrosequencing technique.

During artificial amplification, amplicon length is dictated by the experimental goals.

However, in our experience most genetic identification applications are well served with amplicon sizes less than 160 bp."

Targeted amplicon sequencing relies on having some expectations about the composition of the community that is being studied.

The number of repeats at each locus were deduced from the size of the amplicon produced.

After one extension, semi-amplicon, an amplicon containing the common nucleotide sequence on only the 5' end, is made.

In other cases, the observed amplicon size fell outside of the expected amplicon sizes.

The males are distinguished as having two DNA amplicons present, while females have only a single amplicon.

The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable.

A similar comparison was performed on PCR amplicon probes (Figure 4b).

Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes.

In target amplicon sequencing a phylogenetically informative marker is targeted for sequencing.

In practice, the empirically determined product size was found to sometimes differ from the expected amplicon size by 1- or 2-bp, especially at higher allele numbers.

Therefore the longer the desired PCR amplicon, the more limited the number of intact template molecules will likely be.

You "paint" the amplicon with a double-strand specific dye, included in the PCR mix.

However, PCR involves exponential target amplification, thereby increasing the risk of amplicon cross-contamination.

Next, we compared oligonucleotide-derived ratios with those obtained from PCR amplicon probes (Figure 4c).

At some point during this process, the melting temperature of the amplicon is reached and the two strands of DNA separate or "melt" apart.

Accordingly, each of the corresponding 65 PCR amplicon probes had a positive hybridization signal (threefold above background).

Thus, it is important to assess the amount of DNA degradation resulting from the reaction conditions employed, and consider how this will affect the desired amplicon.