bovine serum albumin

Then he blocked any remaining binding sites in the wells with bovine serum albumin.

The enzyme is supplied by many manufacturers and requires bovine serum albumin to work properly.

Bovine serum albumin may also be used to establish a chiral environment for sulfoxidation.

Transport media should be supplemented with protein, such as bovine serum albumin or gelatin, to a concentration of 0.5% to 1%.

Aesculetin has the ability to quench the inner fluorescence of bovine serum albumin.

For example, a standard curve for protein concentration is often created using known concentrations of bovine serum albumin.

Other studies have implicated neutral endopeptidase and cationic bovine serum albumin as antigens.

Protein content was determined using the Bradford method [ 39 ] with bovine serum albumin as the standard.

Antibodies to bovine serum albumin were present in all patients with type I diabetes of new onset but in only 2.5% of controls.

Blocking was done with 5% bovine serum albumin (Sigma) in PBS.

For detection of phosphotyrosine-containing proteins, filters were blocked with 1% bovine serum albumin (Sigma-Aldrich).

The acid is in complex with fatty-acid free bovine serum albumin and human low-density lipoprotein, added to an enriched mixture of standard cell media.

Serum and antibodies were diluted in freshly-prepared PBS containing 0.5% bovine serum albumin.

Protein concentrations were estimated by the Bradford assay [ 41] with bovine serum albumin as the protein standard.

Antibodies to the relevant peptide sequence in the bovine serum albumin also identified the p 69 antigen from islets on immunoblots.

Bovine serum albumin (BSA)

Supernatants were collected and stored at -20 C. Protein concentration was determined by the method of Lowry using bovine serum albumin as standard.

Early sensitisation to bovine serum albumin through bottle feeding in infancy leaves memory T cells which destroy β cells expressing p 69.

Keyhole limpet hemocyanin (KLH) and bovine serum albumin are two widely used carrier proteins.

Filters were blocked with 3% bovine serum albumin (BSA)(Sigma) at room temperature for at least one hour.

The oligonucleotide used may be synthesised to include a primary n-hexyl amino group at its 5' end, later utilised to attach bovine serum albumin (BSA).

The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum.

The protein concentrations of the samples were determined using the BCA protein assay (Pierce) with bovine serum albumin as the standard.

Nonspecific binding sites were blocked by incubating the NC paper in 2% bovine serum albumin and 3% nonfat milk powder at 37 C for 3 hours.

Each section was preincubated with 2% bovine serum albumin for 1 hour, then incubated overnight at 4 C with the appropriate primary antibody at 1:500 dilution.