immunoprecipitation

One reason for this density issue is its effect on the efficiency of immunoprecipitation.

The remainder of the cell extract was used for immunoprecipitation.

That his experiments indeed seemed to "work" on occasion was probably due to immunoprecipitation.

These reports could perhaps be considered the pioneering work in the field of chromatin immunoprecipitation.

Structural details of the assembly of this complex are not known, only physical association by immunoprecipitation.

Immunoprecipitation with 1 ug of normal rabbit serum served as a negative control.

Chromatin immunoprecipitation allows binding sites of proteins to be identified.

A note of caution also is that immunoprecipitation experiments reveal direct and indirect interactions.

This procedure is essentially an immunoprecipitation (IP) of the protein.

This particular procedure is known as immunoprecipitation.

Immunoprecipitation is quite capable of generating an extremely specific interaction which usually results in binding only the desired protein.

An added benefit of using magnetic beads is that automated immunoprecipitation devices are becoming more readily available.

This crosslinker can also be used to stabilize protein-protein interactions for further analysis by immunoprecipitation.

Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.

One method that is commonly used for identifying the members of protein complexes is immunoprecipitation.

Proteins and protein complexes can be separated, e.g. in immunoprecipitation protocols.

This is why it is important to preclear the sample before the immunoprecipitation is performed.

Polyclonal rabbit anti-ovalbumin was used as a negative control for activation and immunoprecipitation experiments.

By repeating the immunoprecipitation in this way, the researcher verifies that each identified member of the protein complex was a valid identification.

Lyse cells and prepare sample for immunoprecipitation.

The immunoprecipitation assay followed by western blot analysis was as described previously [ 25].

This is due to release of ATF1 during the washing steps of the immunoprecipitation (37).

One of the major technical hurdles with immunoprecipitation is the great difficulty in generating an antibody that specifically targets a single known protein.

The two general methods for immunoprecipitation are the direct capture method and the indirect capture method.

As predicted, both proteins are recognized by antibody specific for PP1α when performing immunoprecipitation or western blotting.